Mosquito saliva induced cutaneous events augment Chikungunya virus replication and disease progression
Autor(es): Agarwal Ankita, Joshi Gaurav, Nagar Durga P, Sharma Ajay K, Sukumaran D, Pant Satish C, Parida Man Mohan, Dash Paban Kumar
Resumo: Chikungunya virus (CHIKV) is transmitted when infected mosquito probes the host skin. While probing, mosquito saliva is expectorated into host skin along with virus which contains cocktail of molecules having anti-hemostatic - immunomodulatory properties. As mosquito saliva is a critical factor during natural arboviral infection, therefore we investigated mosquito saliva induced cutaneous events that modulate CHIKV infection. The effect of mosquito saliva on CHIKV infection was examined through inoculation of suckling mice subcutaneously with either CHIKV alone or uninfected mosquito bite followed by CHIKV. Histopathological evaluation of skin revealed infiltration of transmigrated inflammatory cells. Dermal blood vessels were hyperemic - adnexa showed degenerating lesions. Severe hemorrhage was observed in dermis - hypodermis in mosquito bite+CHIKV group compared to CHIKV group. Analysis of cytokines in skin showed significant downregulation of inflammatory genes like TLR-3, IL-2, IFN-?, TNF-? - IFN-? in mosquito bite+CHIKV group compared to CHIKV group. In contrast, significant upregulation of anti-inflammatory genes like IL-4 - IL-10 was observed. These early events might have been responsible for increased dissemination of CHIKV to serum - peripheral organs as demonstrated through >10-fold higher viremia, antigen localization, cellular infiltration - degenerative changes. Thus mosquito saliva induced early cellular infiltration - associated cytokines augment CHIKV pathogenesis in a mouse model. This mosquito improved CHIKV mouse model simulates the realistic conditions that occur naturally during infected mosquito bite to a host. It will lead to better underst-ing of CHIKV pathobiology - promote the evaluation of novel medical countermeasures against emerging CHIKV.
Palavras-Chave: Chikungunya; Cutaneous immune response; Mosquito saliva; Pathogenesis
Imprenta: Infection, Genetics and Evolution, v. 40, p. 126-135, 2016
Identificador do objeto digital: 10.1016/j.meegid.2016.02.033
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Cytokines ; Chikungunya virus - Immune response ; Chikungunya Virus - Virus
Data de publicação: 2016
