Th17 helper cell and T-cell immunoglobulin and mucin domain 3 involvement in Guillain-Barré syndrome
Autor(es): Liang Song-lan,Wang Wei-zhi,Huang Shuo,Wang Xiao-kun,Zhang Suang,Wu Yun
Resumo: We investigated the involvement of Th17 cells and T-cell immunoglobulin and mucin domain 3 (TIM-3) in Guillain-Barré syndrome (GBS) in comparison to healthy subjects. Peripheral blood samples were obtained from 29 healthy subjects and 29 GBS patients. Peripheral blood mononuclear cells (PBMCs) and CD4(+) T cells were stimulated with anti-CD3 and anti-CD28 mAbs, in the absence or presence of anti-TIM-3 mAb. mRNA levels of TIM-3 and the transcription factor retinoic acid-related orphan receptor ?t (ROR?t) were determined by RT-PCR and were expressed relative to ?-actin mRNA (housekeeping gene). Serum IFN-? and IL-17 levels were determined by ELISA. Compared to controls, relative TIM-3 mRNA levels were lower in both stimulated and unstimulated PBMCs from GBS patients. Unstimulated GBS CD4(+) T cells and GBS CD4+ T cells stimulated with anti-CD3 and CD28 mAbs had higher relative ROR?t mRNA expression compared to controls. GBS CD4(+) T cells secreted significantly more IFN-? and IL-17 in the presence of anti-TIM-3 mAb. GBS patients had (1) higher numbers of Th17, but not Th1 or Th2 cells in peripheral blood and (2) higher serum concentrations of IFN-? and IL-17 compared to controls. TIM-3 may inhibit Th17 cell activation, thereby modulating their cytokine secretion patterns. Th17 cell differentiation, IL-17 levels, and TIM-3 regulation may be involved in the pathogenesis of GBS.
Palavras-Chave: Guillain Barre syndrome; TIM-3; Th17; RORyt; IL-17
Imprenta: Immunopharmacology and Immunotoxicology, v. 34, n. 6, p. 1039-1046, 2012
Identificador do objeto digital: 10.3109/08923973.2012.697469
Descritores: Guillain-Barre Syndrome - Biosynthesis ; Guillain-Barre Syndrome - Cell ; Guillain-Barre Syndrome - Cytopathology ; Guillain-Barre Syndrome - Pathogenesis ; Guillain-Barre Syndrome - Protein synthesis ; Guillain-Barre Syndrome - Proteins ; Guillain-Barre Syndrome - RNA ; Guillain-Barre Syndrome - Cytokines ; Guillain-Barre Syndrome - RT-PCR ; Guillain-Barre Syndrome - Immunology
Data de publicação: 2012
