Validation of an ELISA for the diagnosis of recent Campylobacter infections in Guillain-Barré and reactive arthritis patients
Autor(es): Ang C W,Krogfelt K,Herbrink P,Keijser J,van Pelt W,Dalby T,Kuijf M,Jacobs B C,Bergman M P,Schiellerup P,Visser C E
Resumo: Weeks or months following Campylobacter infection, a small proportion of infected individuals develop Guillain-Barré syndrome (GBS) or reactive arthritis (ReA). Stool culture for Campylobacter is often negative in these patients, and serology is therefore the method of choice for diagnosing a recent infection with Campylobacter. This study developed a capture ELISA system to detect anti-Campylobacter IgA and IgM antibodies indicative of a recent infection. The sensitivity of the assay was 82.0% in uncomplicated Campylobacter enteritis patients, 96.2% in GBS patients who were culture-positive for Campylobacter, and 93.1% in culture-positive ReA patients, with a specificity of 93.0%. The assay allows identification of Campylobacter infection in patients with post-infectious neurological and rheumatological complications.
Palavras-Chave: Arthritis; Campylobacter infections; Diagnosis; ELISA; Guillain-Barré syndrome; Joint pain
Imprenta: Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, v. 13, n. 9, p. 915-922, 2007
Identificador do objeto digital: 10.1111/j.1469-0691.2007.01765.x
Descritores: Guillain-Barre Syndrome - Pathogenesis ; Guillain-Barre Syndrome - Antibodies ; Guillain-Barre Syndrome - Serology ; Guillain-Barre Syndrome - Immunology ; Guillain-Barre Syndrome - Public health
Data de publicação: 2007
