Autor(es): Wolff C,Kruppa T,Dreier J,ter Meulen J

Resumo: The transmissibility of the GB virus C (hepatitis G virus; HGV), a member of the Flaviviridae, by a typical flavivirus vector was investigated. Female mosquitoes of the species Aedes aegypti were fed with HGV-infected human blood and assayed 1, 24, 48, 72 and 96 h after the blood meal for viral RNA, human glyceraldehyde-3-phosphate dehydrogenase mRNA, human beta-actin DNA and A. aegypti actin mRNA by total nucleic acid extraction, reverse transcription and PCR. Viral RNA had already disappeared from nucleic acid extracts 1 h after the blood meal and was not detectable throughout the observation period. Aedes-specific mRNA served as an internal control and was detected in all nucleic acid extracts, whereas human mRNA had disappeared after 24 h, indicating digestion of human cells. From these results we conclude that GB virus C (HGV) cannot replicate in A. aegypti, which is a widespread and competent vector of several other flaviviruses.

Palavras-Chave: Aedes; Flavivirus; GB virus C; Hepatitis G virus; PCR; Virus replication

Imprenta: Microbes and Infection / Institut Pasteur, v. 3, n. 9, p. 683-687, 2001

Identificador do objeto digital: 10.1016/S1286-4579(01)01430-7

Descritores: Aedes aegypti - Cell ; Aedes aegypti - DNA ; Aedes aegypti - Flaviviridae ; Aedes aegypti - Pathogenesis ; Aedes aegypti - RNA ; Aedes aegypti - Viral infections ; Aedes aegypti - RT-PCR ; Aedes aegypti - virus

Data de publicação: 2001

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