Autor(es): Mishra Baijayantimala, Sharma Mirnalini, Pujhari Sujit Kumar, Ratho Radha Kanta, Gopal Dvr Sai, Kumar Cvm Naresh, Sarangi Gita, Chayani Nirupama, Varma Subhash C

Resumo: The reemergence of chikungunya virus (CHIKV) has compounded the already existing dengue problem because of clinical similarities - common vector, dem-ing the need for a rapid - specific diagnosis. Thus, dengue chikungunya multiplex reverse transcriptase-polymerase chain reaction (DCmRT-PCR) was developed - validated for simultaneous detection of dengue - chikungunya viral infections - its utility in virus serotyping. Blood samples from 97 suspected dengue - chikungunya cases - 10 healthy controls were subjected to dengue - chikungunya conventional RT-PCR - DCmRT-PCR. Thirty-one of 97 samples were positive for dengue or chikungunya viral RNA by RT-PCR - DCmRT-PCR with 100% concordance. DCmRT-PCR products were cycle sequenced. Seven dengue virus strains were clustered within genotype III of DENV-3 - 4 within genotype III of DENV-1, whereas chikungunya sequences were clustered within the Central/East African genotype. DCmRT-PCR was found to be a potential rapid test for simultaneous detection of dengue - CHIKV in clinical samples along with dengue serotyping.

Palavras-Chave: Multiplex RT-PCR; Dengue; Chikungunya; Diagnosis; Serotyping

Imprenta: Diagnostic Microbiology and Infectious Disease, v. 71, n. 2, p. 118-125, 2011

Identificador do objeto digital: 10.1016/j.diagmicrobio.2011.06.020

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Public health

Data de publicação: 2011

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