Unique epitopes recognized by antibodies induced in Chikungunya virus-infected non-human primates: Implications for the study of immunopathology and vaccine development
Autor(es): Kam Yiu-Wing, Lee Wendy W L, Simarmata Diane, Le Grand Roger, Tolou Hugues, Merits Andres, Roques Pierre, Ng Lisa F P
Resumo: Chikungunya virus (CHIKV) is an Alphavirus that causes chronic - incapacitating arthralgia in humans. Although patient cohort studies have shown the production of CHIKV specific antibodies, the fine specificity of the antibody response against CHIKV is not completely defined. The macaque model of CHIKV infection was established due to limitations of clinical specimens. More importantly, its close relation to humans will allow the study of chronic infection - further identify important CHIKV targets. In this study, serum samples from CHIKV-infected macaques collected at different time-points post infection were used to characterize the antibody production pattern - kinetics. Results revealed that anti-CHIKV antibodies were neutralizing - the E2 glycoprotein, Capsid, nsP1, nsP3 - nsP4 proteins were targets of the anti-CHIKV antibody response in macaques. Furthermore, linear B-cell epitopes recognized by these anti-CHIKV antibodies were identified, - mapped to their structural localization. This characterizes the specificity of anti-CHIKV antibody response in macaques - further demonstrates the importance of the different regions in CHIKV-encoded proteins in the adaptive immune response. Information from this study provides critical knowledge that will aid in the underst-ing of CHIKV infection - immunity, vaccine design, - pre-clinical efficacy studies.
Imprenta: PloS One, v. 9, n. 4, p. e95647, 2014
Identificador do objeto digital: 10.1371/journal.pone.0095647
Descritores: Chikungunya virus - Biochemistry ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Immune response ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Immune response ; Chikungunya virus - Immunopathology ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology
Data de publicação: 2014
