The C-terminal repeat domains of nsP3 from the Old World alphaviruses bind directly to G3BP
Autor(es): Panas Marc D, Ahola Tero, McInerney Gerald M
Resumo: The Old World alphaviruses block stress granule assembly by sequestration of RasGAP SH3-domain binding protein (G3BP). Here, we show that the proline-rich sequences in the hypervariable domain of nonstructural protein 3 (nsP3) of both Semliki Forest virus - Chikungunya virus were dispensable for binding to G3BP. nsP3 variants with or without this domain colocalized with G3BP. Furthermore, we show that the C-terminal repeat motifs of nsP3 were sufficient for G3BP binding.
Imprenta: Journal of Virology, v. 88, n. 10, p. 5888-5893, 2014
Identificador do objeto digital: 10.1128/JVI.00439-14
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Proteins ; Chikungunya Virus - Virus
Data de publicação: 2014
