The C-terminal domain of Chikungunya virus nsP2 independently governs viral RNA replication, cytopathicity, and inhibition of interferon signaling
Autor(es): Fros Jelke J, van der Maten Erika, Vlak Just M, Pijlman Gorben P
Resumo: Alphavirus nonstructural protein 2 (nsP2) has pivotal roles in viral RNA replication, host cell shutoff, - inhibition of antiviral responses. Mutations that individually rendered other alphaviruses noncytopathic were introduced into chikungunya virus nsP2. Results show that (i) nsP2 mutation P718S only in combination with KR649AA or adaptive mutation D711G allowed noncytopathic replicon RNA replication, (ii) prohibiting nsP2 nuclear localization abrogates inhibition of antiviral interferon-induced JAK-STAT signaling, - (iii) nsP2 independently affects RNA replication, cytopathicity, - JAK-STAT signaling.
Imprenta: Journal of Virology, v. 87, n. 18, p. 10394-10400, 2013
Identificador do objeto digital: 10.1128/JVI.00884-13
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Cytokines ; Chikungunya Virus - Virus
Data de publicação: 2013
