Autor(es): Huang Yan-Jang S, Hsu Wei-Wen, Higgs Stephen, Vanlandingham Dana L

Resumo: In late 2013, chikungunya virus (CHIKV) was introduced to the New World - large outbreaks occurred in the Caribbean isl-s causing over a million suspected - over 20,000 laboratory-confirmed cases. Serological analysis is an essential component for the diagnosis of CHIKV infection together with virus isolation - detection of viral nucleic acid. Demonstrating virus neutralizing by serum antibodies in a plaque reduction neutralization test (PRNT) is the gold st-ard of all serological diagnostic assays. Prior to the testing, heat inactivation of serum at 56°C for 30 min is required for the inactivation of complement activity - adventitious viruses. The presence of adventitious contaminating viruses may interfere with the results by leading to a higher number of plaques on the monolayers - subsequent false-negative results. This procedure is widely accepted for the inactivation of flaviviruses - alphaviruses. In this study, the thermostability of CHIKV was evaluated. Heat inactivation at 56°C for 30 min was demonstrated to be insufficient for the complete removal of infectious CHIKV virions present in the samples. This thermotolerance of CHIKV could compromise the accuracy of serum tests, - therefore longer treatment for greater than 120 min is recommended.

Palavras-Chave: Chikungunya virus; Heat inactivation; Thermostability

Imprenta: Vector Borne and Zoonotic Diseases, v. 15, n. 11, p. 674-677, 2015

Identificador do objeto digital: 10.1089/vbz.2015.1795

Descritores: Chikungunya virus - Antibodies ; Chikungunya virus - Serological diagnosis ; Chikungunya Virus - Virus

Data de publicação: 2015