Rapid detection and quantification of Chikungunya virus by a one-step reverse transcription polymerase chain reaction real-time assay
Autor(es): Carletti Fabrizio, Bordi Licia, Chiappini Roberta, Ippolito Giuseppe, Sciarrone Maria R, Capobianchi Maria R, Di Caro Antonino, Castilletti Concetta
Resumo: Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus. A large outbreak of CHIKV disease occurred in 2005 in the Indian Ocean Isl-s. Many cases have been imported in European countries. Laboratory confirmation of suspected cases is m-atory for control measures during an outbreak. We report a novel, real-time, reverse transcription-polymerase chain reaction (RT-PCR) for the nonstructural protein 1 region that can quantify a wide range of viral RNA concentrations. This assay was validated by in vitro experiments in which interferon-alpha, a well-known virus inhibitor, showed a dose-dependent inhibition of virus replication on Vero cells that was assessed by viral infectivity - viral RNA production. This new real-time RT-PCR was used to measure viral load in serum samples from cases recently imported to Italy, - may be a useful tool in rapid detection of CHIKV - monitoring the extent of viral replication in patients.
Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 77, n. 3 , p. 521-524, 2007
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Public health
Data de publicação: 2007
