Photochemical inactivation of Chikungunya virus in plasma and platelets using the Mirasol pathogen reduction technology system
Autor(es): Vanlandingham Dana L, Keil Shawn D, Horne Kate McElroy, Pyles Richard, Goodrich Raymond P, Higgs Stephen
Resumo: Chikungunya virus (CHIKV) is a reemerging mosquito-borne virus that has been responsible for a number of large-scale epidemics as well as imported cases covering a wide geographical range. As a blood-borne virus capable of mounting a high-titer viremia in infected humans, CHIKV was included on a list of risk agents for transfusion - organ transplant by the AABB Transfusion-Transmitted Diseases Committee. Therefore, we evaluated the ability of the Mirasol pathogen reduction technology (PRT) system (CaridianBCT Biotechnologies) to inactivate live virus in contaminated plasma - platelet (PLT) samples. Plasma, PLTs, - phosphate-buffered saline controls were spiked with CHIKV - treated with riboflavin - varying doses of ultraviolet (UV) light using the Mirasol PRT system. Samples were tested before - after treatment for cytotoxicity, interference, - virus titer by titration - quantitative real-time reverse transcription-polymerase chain reaction. A significant reduction in CHIKV titer of greater than 99% was recorded after treatment of plasma or PLTs with the Mirasol PRT system, - the titer reduction was directly proportional to the UV dose delivered to the samples. No cytotoxicity of interference was observed in any sample at any treatment dose. These data indicate that the Mirasol PRT system efficiently inactivated live CHIKV in plasma - PLTs - could therefore potentially be used to prevent CHIKV transmission through the blood suply.
Imprenta: Transfusion, v. 53, n. 2, p. 284-290, 2013
Identificador do objeto digital: 10.1111/j.1537-2995.2012.03717.x
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya virus - Real Time PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health
Data de publicação: 2013
