Autor(es): Goh Lucas Y H, Hobson-Peters Jody, Prow Natalie A, Gardner Joy, Bielefeldt-Ohmann Helle, Pyke Alyssa T, Suhrbier Andreas, Hall Roy A

Resumo: Chikungunya virus (CHIKV) recently caused the largest epidemic ever recorded for this virus involving an estimated 1.4-6.5million cases, with imported cased reported in over 40 countries. The number of monoclonal antibodies specific for this re-emerging alphavirus is currently limited. Herein we describe the generation - characterisation of five monoclonal antibodies specific for the E2 glycoprotein of CHIKV. The antibodies detected a range of CHIKV isolates in several assays including ELISA, Western blot, immunofluorescence assay (IFA) - immunohistochemistry (IHC) without evidence of cross-reactivity with other alphaviruses. Four antibodies also neutralised CHIKV in vitro, two of which provided complete protection against arthritis in a CHIKV mouse model when administered prior to infection. Given the current shortage of widely available reagents for CHIKV, these specific antibodies will be useful not only in research, but may also provide the basis for new diagnostics - treatments.

Palavras-Chave: Chikungunya virus; Diagnostic; Disease protection; ELISA; Immunohistochemistry; Monoclonal antibodies

Imprenta: Clinical Immunology, v. 149, n. 3, p. 487-497, 2013

Identificador do objeto digital: 10.1016/j.clim.2013.10.004

Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Protein synthesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Epidemic ; Chikungunya virus - Immunology

Data de publicação: 2013