Nanopatch-targeted skin vaccination against West Nile Virus and Chikungunya virus in mice
Autor(es): Prow Tarl W, Chen Xianfeng, Prow Natalie A, Fernando Germain J P, Tan Cindy S E, Raphael Anthony P, Chang David, Ruutu Merja P, Jenkins Derek W K, Pyke Alyssa, Crichton Michael L, Raphaelli Kristin, Goh Lucas Y H, Frazer Ian H, Roberts Michael S, Gardner Joy, Khromykh Alexander A, Suhrbier Andreas, Hall Roy A, Kendall Mark A F
Resumo: The 'Nanopatch' (NP) comprises arrays of densely packed projections with a defined geometry - distribution designed to physically target vaccines directly to thous-s of epidermal - dermal antigen presenting cells (APCs). These miniaturized arrays are two orders of magnitude smaller than st-ard needles-which deliver most vaccines-- are also much smaller than current microneedle arrays. The NP is dry-coated with antigen, adjuvant, -/or DNA payloads. After the NP was pressed onto mouse skin, a protein payload co-localized with 91.4 + or - 4.1 APC mm(-2) (or 2925 in total) representing 52% of the delivery sites within the NP contact area, agreeing well with a probability-based model used to guide the device design; it then substantially increases as the antigen diffuses in the skin to many more cells. APC co-localizing with protein payloads rapidly disappears from the application area, suggesting APC migration. The NP also delivers DNA payloads leading to cutaneous expression of encoded proteins within 24 h. The efficiency of NP immunization is demonstrated using an inactivated whole chikungunya virus vaccine - a DNA-delivered attenuated West Nile virus vaccine. The NP thus offers a needle-free, versatile, highly effective vaccine delivery system that is potentially inexpensive - simple to use.
Imprenta: Small, v. 6, n. 16, p. 1776-1784, 2010
Identificador do objeto digital: 10.1002/smll.201000331
Descritores: Chikungunya virus - Cell ; Chikungunya virus - DNA ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2010
