Multiplex real-time RT-PCR for detecting Chikungunya virus and Dengue virus
Autor(es): Pongsiri Piyathida, Praianantathavorn Kesmanee, Theamboonlers Apiradee, Payungporn Sunchai, Poovorawan Yong
Resumo: To develop diagnostic test for detection chikungunya virus (CHIKV - Dengue virus (DENV) infection. We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify - differentiate CHIKV - DENV infection by single-step multiplex real-time RT-PCR. The assay's sensitivity was 97.65%, specificity was 92.59% - accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus. This rapid - reliable assay provides a means for simultaneous early diagnosis of CHIKV - DENV in a single-step reaction.
Palavras-Chave: Multiplex; Real-time RT-PCR; Chikungunya virus; Dengue virus
Imprenta: Asian Pacific Journal of Tropical Medicine, v. 5, n. 5, p. 342-346, 2012
Identificador do objeto digital: 10.1016/S1995-7645(12)60055-8
Descritores: Chikungunya virus - DNA ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Public health
Data de publicação: 2012
