Human keratinocytes restrict Chikungunya virus replication at a post-fusion step
Autor(es): Bernard Eric, Hamel Rodolphe, Neyret Aymeric, Ekchariyawat Peeraya,Molès Jean-Pierre, Simmons Graham, Chazal Nathalie, Desprès Philippe, Missé Dorothée, Briant Laurence
Resumo: Transmission of chikungunya virus (CHIKV) to humans is initiated by puncture of the skin by a blood-feeding Aedes mosquito. Despite the growing knowledge accumulated on CHIKV, the interplay between skin cells - CHIKV following inoculation still remains unclear. In this study we questioned the behavior of human keratinocytes, the predominant cell population in the skin, following viral challenge. We report that CHIKV rapidly elicits an innate immune response in these cells leading to the enhanced transcription of type I/II - type III interferon genes. Concomitantly, we show that despite viral particles internalization into Rab5-positive endosomes - efficient fusion of virus - cell membranes, keratinocytes poorly replicate CHIKV as attested by absence of nonstructural proteins - genomic RNA synthesis. Accordingly, human keratinocytes behave as an antiviral defense against CHIKV infection rather than as a primary targets for initial replication. This picture significantly differs from that reported for Dengue - West Nile mosquito-borne viruses.
Palavras-Chave: Chikungunya; Innate immunity; Keratinocytes; Replication
Imprenta: Virology, v. 476, p. 1-10, 2015
Identificador do objeto digital: 10.1016/j.virol.2014.11.013
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Cytokines ; Chikungunya virus - Immune response ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Immunology
Data de publicação: 2015
