Autor(es): Dudha Namrata, Rana Jyoti, Rajasekharan Sreejith, Gabrani Reema, Gupta Amita, Chaudhary Vijay Kumar, Gupta Sanjay

Resumo: The envelope proteins of Chikungunya virus (CHIKV) are known to play crucial roles in viral infection - spread. Although the role of envelope proteins in viral infection has been studied, the cellular interactors of these proteins are still elusive. In the present study, the ectodomains of CHIKV envelope proteins (E1 - E2) have been used for a high throughput yeast two-hybrid (Y2H) screening to identify the interacting host protein partners. Following a comparative analysis between the viral-host protein interaction data generated from Y2H - computational approach, five host proteins interacting with E1 - three host proteins interacting with E2 common to both datasets were identified. These associations were further verified independently by pull down - protein interaction ELISA. The identified interactions shed light on the possible cellular machinery that CHIKV might be employing during viral entry, trafficking, - evasion of immune system.

Palavras-Chave: Chikungunya virus; Envelope proteins; Virus-host interaction

Imprenta: Virus Genes, v. 50, n. 2, p. 200-209, 2015

Identificador do objeto digital: 10.1007/s11262-014-1161-x

Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever

Data de publicação: 2015

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