Autor(es): Saswat Tanuja, Kumar Abhishek, Kumar Sameer, Mamidi Prabhudutta, Muduli Sagarika, Debata Nagen Kumar, Pal Niladri Shekhar, Pratheek B M, Chattopadhyay Subhasis, Chattopadhyay Soma

Resumo: Dengue viral (DENV) infection is endemic in different parts of India - because of similar primary signs - symptoms, Chikungunya virus (CHIKV) is mostly undiagnosed. Hence, we investigated 204 suspected Dengue cases in a hospital based cross-sectional study in Odisha, India in 2013. It was observed that 50 samples were positive for DENV only, 28 were positive for CHIKV only - interestingly, 28 patients were co-infected with both DENV - CHIKV. Additionally, a total of 18 confirmed Dengue samples from Maharashtra, India were screened for CHIKV - out of those, 15 were co-infected. All CHIKV strains were of East Central South African (ECSA) type - serotype 2 (genotype IV) was predominant in the DENV samples. Additionally, Dengue serotype 1 - 3 were also detected during this time. Further, sequence analysis of E1 gene of CHIKV strains revealed that two substitution mutations (M269V - D284E) were observed in almost 50% strains - they were from co-infected patients. Similarly, sequence analysis of C-prM gene showed the presence of five substitution mutations, (G70S, L72F, N90S, S93N - I150L) in all serotype 1 - two consistent mutations (A101V - V112A) in serotype 2 Dengue samples. Together, it appears that a significantly high number of dengue patients (43, 44.8%) were co-infected with DENV - CHIKV during this study. This emphasizes the need of a routine diagnosis of CHIKV along with DENV for febrile patients. This will be useful in early - proper recognition of infecting pathogen to study the correlation of clinical symptoms with single or co-infection which will ultimately help to implement proper patient care in future.

Palavras-Chave: Alphavirus; Chikungunya virus; Co-infection; Dengue virus; Epidemiology; Flavivirus; Outbreak

Imprenta: Infection, Genetics and Evolution, v. 35, p. 134-141, 2015

Identificador do objeto digital: 10.1016/j.meegid.2015.08.006

Descritores: Chikungunya virus - Cytopathology ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemiology

Data de publicação: 2015