Autor(es): Owino Eunice A, Sang Rosemary, Sole Catherine L, Pirk Christian, Mbogo Charles, Torto Baldwyn

Resumo: Methods currently used in sampling adult Aedes aegypti, the main vector of dengue - chikungunya viruses are limited for effective surveillance of the vector - accurate determination of the extent of virus transmission during outbreaks - inter - epidemic periods. Here, we document the use of natural human skin odours in baited traps to improve sampling of adult Ae. aegypti in two different endemic areas of chikungunya - dengue in Kenya - Kilifi - Busia Counties. The chemistry of the volatiles released from human odours - the Biogent (BG)-commercial lure were also compared. Cotton socks - T-shirts were used to obtain natural human skin volatiles from the feet - trunk of three volunteers (volunteers 1 - 2 in Kilifi - volunteers 2 - 3 in Busia). Using Latin square design, we compared the efficacies of BG sentinel traps baited with carbon dioxide plus (a) no bait, (b) human feet volatiles, (c) human trunk volatiles each against (c) a control (Biogent commercial lure) at the two sites. Coupled gas chromatography-mass spectrometry (GC-MS) was used to identify - compare c-idate attractants released by the commercial lure - human odours. Ae. aegypti captured in the trap baited with feet odours from volunteer 2 - trunk odours from the same volunteer were significantly higher than in the control trap in Busia - Kilifi respectively, [IRR = 5.63, 95% CI: 1.15 - 28.30, p = 0.030] - [IRR = 3.99, 95% CI: 0.95-16.69, p = 0.049]. At both sites, Ae. aegypti captures in traps baited with either the feet or trunk odours from volunteers 1 - 3 were not significantly different from the control. Major qualitative differences were observed between the chemical profiles of human odours - the commercial BG-lure. Aldehydes, fatty acids - ketones dominated human odour profiles, whereas the BG-lure released mainly hexanoic acid. Our results suggest that additional c-idate attractants are present in human skin volatiles which can help to improve the efficacy of lures for trapping - surveillance of Ae. aegypti.

Palavras-Chave: Aedes aegypti; Dengue; Chikungunya; Human odour; Mosquito; Traps

Imprenta: Parasites & Vectors, v. 7, p. 451, 2014

Identificador do Objeto Digital: 10.1186/1756-3305-7-451

Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health

Data de Publicação: 2014