Evidence of experimental vertical transmission of emerging novel ECSA genotype of Chikungunya virus in Aedes aegypti
Autor(es): Agarwal Ankita, Dash Paban Kumar, Singh Anil Kumar, Sharma Shashi, Gopalan Natarajan, Rao Putcha Venkata Lakshmana, Parida Man Mohan, Reiter Paul
Resumo: Chikungunya virus (CHIKV) has emerged as one of the most important arboviruses of public health significance in the past decade. The virus is mainly maintained through human-mosquito-human cycle. Other routes of transmission - the mechanism of maintenance of the virus in nature are not clearly known. Vertical transmission may be a mechanism of sustaining the virus during inter-epidemic periods. Laboratory experiments were conducted to determine whether Aedes aegypti, a principal vector, is capable of vertically transmitting CHIKV or not. Female Ae. aegypti were orally infected with a novel ECSA genotype of CHIKV in the 2nd gonotrophic cycle. On day 10 post infection, a non-infectious blood meal was provided to obtain another cycle of eggs. Larvae - adults developed from the eggs obtained following both infectious - non-infectious blood meal were tested for the presence of CHIKV specific RNA through real time RT-PCR. The results revealed that the larvae - adults developed from eggs derived from the infectious blood meal (2nd gonotrophic cycle) were negative for CHIKV RNA. However, the larvae - adults developed after subsequent non-infectious blood meal (3rd gonotrophic cycle) were positive with minimum filial infection rates of 28.2 (1?35.5) - 20.2 (1?49.5) respectively. This study is the first to confirm experimental vertical transmission of emerging novel ECSA genotype of CHIKV in Ae. aegypti from India, indicating the possibilities of occurrence of this phenomenon in nature. This evidence may have important consequence for survival of CHIKV during adverse climatic conditions - inter-epidemic periods.
Imprenta: PLoS Neglected Tropical Diseases, v. 8, n. 7, p. e2990, 2014
Identificador do objeto digital: 10.1371/journal.pntd.0002990
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health
Data de publicação: 2014
