Differentiation of group A arboviruses chikungunya, mayaro, and semliki forest by the fluorescent antibody technique
Autor(es): Buckley S M, Clarke D H
Resumo: A conjugate subjected to diethyl-aminoethyl-cellulose chromatography and gradient elution to eliminate both underconjugated and overconjugated antibody molecules has been used to localize specifically Chikungunya, Mayaro, and Semliki Forest viruses in HeLa cells. Immunofluorescence was present in the cytoplasm only and was diffuse in nature; nonspecific staining was negligible. The three viruses, closely related members of arbovirus group A, were readily distinguishable by the indirect fluorescent antibody (FA) technique. The FA titers of homologous systems were reproducible and uniformly higher than the FA titers obtained in heterologous systems.
Imprenta: Proceedings of the Society for Experimental Biology and Medicine, v. 135, n. 2, p. 533-539, 1970
Identificador do objeto digital: 10.3181/00379727-135-35089a
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Pathogenesis ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology
Data de publicação: 1970
