Autor(es): Lohachanakul Jindarat, Phuklia Weerawat, Thannagith Montri, Thongsakulprasert Tipparat, Smith Duncan R, Ubol Sukathida

Resumo: In addition to fever, rash, - arthralgia/arthritis, myalgia is another dominant symptom in Chikungunya virus (CHIKV) infection. How CHIKV induces myalgia is unclear. To better underst- the viral factors involved in CHIKV-induced myalgia, CHIKVs were isolated from patients with - without myalgia designated myalgia-CHIKV - mild-CHIKV, respectively. The response of myoblasts to infection by the two groups of clinical isolates of CHIKV was investigated. Both groups of CHIKV replicated well in primary human myoblasts. However, the myalgia-CHIKVs replicated to a higher titer - caused the death of infected myoblast more rapidly than the mild-CHIKVs. CHIKV-infected myoblasts increased production of four out of five inflammatory cytokines examined (MCP-1, IP-10, MIP-1?, - IL-8) in comparison to mock-infected cells. Comparison between the myoblast inflammatory cytokine responses showed that myalgia-CHIKVs were stronger activators of cytokines than mild-CHIKVs. This means that recent epidemic strains of CHIKV exhibited different degrees of myoblast permissiveness as evidenced by differences in the ability to replicate - to stimulate inflammatory responses in myoblasts. This data suggest that the myopathic syndrome in recent epidemics is dependent upon the strain of CHIKV.

Palavras-Chave: CHIKVs; Chikungunya; Myalgia; Myoblast

Imprenta: Journal of Medical Virology, v. 87, n. 5, p. 733-739, 2015

Identificador do objeto digital: 10.1002/jmv.24081

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology

Data de publicação: 2015

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