Development of novel antibodies against non-structural proteins nsP1, nsP3 and nsP4 of Chikungunya virus: Potential use in basic research
Autor(es): Kumar Sameer, Mamidi Prabhudutta, Kumar Abhishek, Basantray Itishree, Bramha Umarani, Dixit Anshuman, Maiti Prasanta Kumar, Singh Sujay, Suryawanshi Amol Ratnakar, Chattopadhyay Subhasis, Chattopadhyay Soma
Resumo: Chikungunya virus (CHIKV) has reemerged recently as an important pathogen, causing several large epidemics worldwide. This necessitates the development of better reagents to underst- its biology - to establish effective - safe control measures. The present study describes the development - characterization of polyclonal antibodies (pAbs) against synthetic peptides of CHIKV non-structural proteins (nsPs; nsP1, nsP3 - nsP4). The reactivity of these pAbs was demonstrated by ELISA - Western blot. Additionally, in vitro infection studies in a mammalian system confirmed that these pAbs are highly sensitive - specific for CHIKV nsPs, as these proteins were detected very early during viral replication. Homology analysis of the selected epitope sequences revealed that they are conserved among all of the CHIKV strains of different genotypes, while comparison with other alphavirus sequences showed that none of them are 100% identical to the epitope sequences (except Onyong-nyong - Igbo Ora viruses, which show 100% identity to the nsP4 epitope). Interestingly, two different forms of CHIKV nsP1 - three different forms of nsP3 were detected in Western blot analysis during infection; however, further experimental investigations are required to confirm their identity. Also, the use of these antibodies demonstrated faster - enhanced expression profiles of all CHIKV nsPs in 2006 Indian outbreak strains when compared to the CHIKV prototype strain, suggesting the epidemic potential of the 2006 isolate. Accordingly, it can be suggested that the pAbs reported in this study can be used as sensitive - specific tools for experimental investigations of CHIKV replication - infection.
Imprenta: Archives of Virology, v. 160, n. 11, p. 2749-2761, 2015
Identificador do objeto digital: 10.1007/s00705-015-2564-2
Descritores: Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya Virus - Virus ; Chikungunya virus - Epidemic
Data de publicação: 2015
