Autor(es): Klein F, Mahlandt B G, Cockey R R, Lincoln R E

Resumo: Simple - efficient methods for concentrating Rift Valley fever (RVF) virus - chikungunya (CHIK) virus are described. Ammonium sulfate, potassium sulfate, or alcohol was used as a precipitating agent - the precipitate was resuspended to volumes suitable for further processing - purification. The methods permitted concentration of live RVF virus - CHIK virus about 100-fold with negligible losses of virus. RVF virus retained a high level of infectivity with potassium aluminum sulfate - alcohol, but CHIK virus retained a higher infectivity level with ammonium sulfate than with potassium aluminum sulfate. The data indicate that serum plays an important role in the concentration of both viruses, at least when the sulfate methods are used.

Imprenta: Applied Microbiology, v. 20, n. 3, p. 346-350, 1970

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever

Data de publicação: 1970

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