Autor(es): Sourisseau Marion, Schilte Clémentine, Casartelli Nicoletta, Trouillet Céline, Guivel-Benhassine Florence, Rudnicka Dominika, Sol-Foulon Nathalie, Le Roux Karin, Prevost Marie-Christine, Fsihi Hafida, Frenkiel Marie-Pascale, Blanchet Fabien, Afonso Philippe V, Ceccaldi Pierre-Emmanuel, Ozden Simona, Gessain Antoine, Schuffenecker Isabelle, Verhasselt Bruno, Zamborlini Alessia, Saïb Ali, Rey Felix A, Arenzana-Seisdedos Fernando, Desprès Philippe, Michault Alain, Albert Matthew L, Schwartz Olivier

Resumo: An unprecedented epidemic of chikungunya virus (CHIKV) infection recently started in countries of the Indian Ocean area, causing an acute - painful syndrome with strong fever, asthenia, skin rash, polyarthritis, - lethal cases of encephalitis. The basis for chikungunya disease - the tropism of CHIKV remain unknown. Here, we describe the replication characteristics of recent clinical CHIKV strains. Human epithelial - endothelial cells, primary fibroblasts -, to a lesser extent, monocyte-derived macrophages, were susceptible to infection - allowed viral production. In contrast, CHIKV did not replicate in lymphoid - monocytoid cell lines, primary lymphocytes - monocytes, or monocyte-derived dendritic cells. CHIKV replication was cytopathic - associated with an induction of apoptosis in infected cells. Chloroquine, bafilomycin-A1, - short hairpin RNAs against dynamin-2 inhibited viral production, indicating that viral entry occurs through pH-dependent endocytosis. CHIKV was highly sensitive to the antiviral activity of type I - II interferons. These results provide a general insight into the interaction between CHIKV - its mammalian host.

Imprenta: PLoS Pathogens, v. 3, n. 6, p. e89, 2007

Identificador do objeto digital: 10.1371/journal.ppat.0030089

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Epidemiology

Data de publicação: 2007

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