Characterization of Chikungunya virus infection in human neuroblastoma SH-SY5Y cells: Role of apoptosis in neuronal cell death
Autor(es): Dhanwani R, Khan M, Bhaskar A S B, Singh Rajpriya, Patro I K, Rao P V L, Parida M M
Resumo: Chikungunya infection is characterized by fever, rash - arthritis. The disease pathogenesis is still poorly understood. Hence, unveiling the molecular mechanisms that govern the survival - death of neuronal cells infected by Chikungunya virus (CHIKV) was the particular interest of this study. Human neuroblastoma SH-SY5Y cells infected with CHIKV showed characteristic features of apoptosis with activation of caspase-3, cleavage of PARP - translocation of Cyt-c. Cells also showed a loss in the intracellular level of GSH - an increase in the lipid peroxidation of the infected cells with the increasing time of infection, which indicated the involvement of oxidative stress in Chikungunya infection. There was observed a gradual decrease in the fold change of antioxidant enzymes - an increase in the fold change of pro-inflammatory cytokines. This study suggested the implication of virus induced apoptosis in disease pathogenesis which may give a fresh insight for CHIKV induced neuronal cell damage - antiviral therapeutics.
Palavras-Chave: Chikungunya; Apoptosis; Neuronal cells; Oxidative stress
Imprenta: Virus Research, v. 163, n. 2, p. 563-572, 2012
Identificador do objeto digital: 10.1016/j.virusres.2011.12.009
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever
Data de publicação: 2012
