Characterization of Chikungunya virus induced host response in a mouse model of viral myositis
Autor(es): Dhanwani Rekha, Khan Mohsin, Lomash Vinay, Rao Putcha Venkata Lakshmana, Ly Hinh, Parida Manmohan
Resumo: While a number of studies have documented the persistent presence of chikungunya virus (CHIKV) in muscle tissue with primary fibroblast as the preferable cell target, little is known regarding the alterations that take place in muscle tissue in response to CHIKV infection. Hence, in the present study a permissive mouse model of CHIKV infection was established - characterized in order to underst- the pathophysiology of the disease. The two dimensional electrophoresis of muscle proteome performed for differential analysis indicated a drastic reprogramming of the proteins from various classes like stress, inflammation, cytoskeletal, energy - lipid metabolism. The roles of the affected proteins were explained in relation to virus induced myopathy which was further supported by the histopathological - behavioural experiments proving the lack of hind limb coordination - other loco-motor abnormalities in the infected mice. Also, the level of various pro-inflammatory mediators like IL-6, MCP-1, Rantes - TNF-? was significantly elevated in muscles of infected mice. Altogether this comprehensive study of characterizing CHIKV induced mouse myopathy provides many potential targets for further evaluation - biomarker study.
Imprenta: PloS One, v. 9, n. 3, p. e92813, 2014
Identificador do objeto digital: 10.1371/journal.pone.0092813
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Proteome ; Chikungunya virus - Cytokines ; Chikungunya virus - Inflammation ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology
Data de publicação: 2014
