CD8+ T cells control Ross River virus infection in musculoskeletal tissues of infected mice
Autor(es): Burrack Kristina S, Montgomery Stephanie A, Homann Dirk, Morrison Thomas E
Resumo: Ross River virus (RRV), chikungunya virus, - related alphaviruses cause debilitating polyarthralgia - myalgia. Mouse models of RRV - chikungunya virus have demonstrated a role for the adaptive immune response in the control of these infections. However, questions remain regarding the role for T cells in viral control, including the magnitude, location, - dynamics of CD8(+) T cell responses. To address these questions, we generated a recombinant RRV expressing the H-2(b)-restricted glycoprotein 33 (gp33) determinant derived from the glycoprotein of lymphocytic choriomeningitis virus. Using tetramers, we tracked gp33-specific CD8(+) T cells during RRV-lymphocytic choriomeningitis virus infection. We found that acute RRV infection induces activation of CD8(+) T cell responses in lymphoid - musculoskeletal tissues that peak from 10-14 d postinoculation, suggesting that CD8(+) T cells contribute to control of acute RRV infection. Mice genetically deficient for CD8(+) T cells or wild-type mice depleted of CD8(+) T cells had elevated RRV loads in skeletal muscle tissue, but not joint-associated tissues, at 14 d postinoculation, suggesting that the ability of CD8(+) T cells to control RRV infection is tissue dependent. Finally, adoptively transferred T cells were capable of reducing RRV loads in skeletal muscle tissue of Rag1(-/-) mice, indicating that T cells can contribute to the control of RRV infection in the absence of B cells - Ab. Collectively, these data demonstrate a role for T cells in the control of RRV infection - suggest that the antiviral capacity of T cells is controlled in a tissue-specific manner.
Imprenta: The Journal of Immunology, v. 194, n. 2, p. 678-689, 2015
Identificador do objeto digital: 10.4049/jimmunol.1401833
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Immune response ; Chikungunya virus - T lymphocytes ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology
Data de publicação: 2015
