Administration of E2 and NS1 siRNAs inhibit Chikungunya virus replication in vitro and protects mice infected with the virus
Autor(es): Parashar Deepti, Paingankar Mandar S, Kumar Satyendra, Gokhale Mangesh D, Sudeep A B, Shinde Sapana B, Arankalle V A
Resumo: Chikungunya virus (CHIKV) has reemerged as a life threatening pathogen - caused large epidemics in several countries. So far, no licensed vaccine or effective antivirals are available - the treatment remains symptomatic. In this context, development of effective - safe prophylactics - therapeutics assumes priority. We evaluated the efficacy of the siRNAs against ns1 - E2 genes of CHIKV both in vitro - in vivo. Four siRNAs each, targeting the E2 (Chik-1 to Chik-4) - ns1 (Chik-5 to Chik-8) genes were designed - evaluated for efficiency in inhibiting CHIKV growth in vitro - in vivo. Chik-1 - Chik-5 siRNAs were effective in controlling CHIKV replication in vitro as assessed by real time PCR, IFA - plaque assay. CHIKV replication was completely inhibited in the virus-infected mice when administered 72 hours post infection. The combination of Chik-1 - Chik-5 siRNAs exhibited additive effect leading to early - complete inhibition of virus replication. These findings suggest that RNAi capable of inhibiting CHIKV growth might constitute a new therapeutic strategy for controlling CHIKV infection - transmission.
Imprenta: PLoS Neglected Tropical Diseases, v. 7, n. 9, p. e2405, 2013
Identificador do objeto digital: 10.1371/journal.pntd.0002405
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - Real Time PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Vaccine ; Chikungunya virus - Epidemic
Data de publicação: 2013
