Acute meningoencephalitis associated with echovirus 9 infection in Sri Lanka, 2009
Autor(es): Danthanarayana Nayomi, Williams David T, Williams Simon Hedley, Thevanesam Vasanthi, Speers David J, Fernando M S S
Resumo: The aetiology of acute meningoencephalitis in Sri Lankan children - adults is poorly understood. This study was carried out to determine pathogens responsible for meningoencephalitis in Sri Lanka. A hospital-based cross-sectional study was performed using cerebrospinal fluid samples (22 adult - 17 pediatric) collected from August to December 2009 from patients clinically diagnosed with acute meningoencephalitis at two tertiary care hospitals in Sri Lanka. Routine microbiology for bacterial pathogens together with in-house RT-PCR - PCR assays for the detection of dengue viruses, Japanese encephalitis virus, West Nile virus, chikungunya virus, enteroviruses, mumps virus, measles virus, herpes simplex viruses types 1 - 2, - varicella zoster virus were performed. Bacterial pathogens were not isolated from any patient specimens. However, from nine of the paediatric patients aged 1 month to 10 years (mean age 5.2 years) echovirus 9 (E-9; family Picornaviridae, genus Enterovirus,species Enterovirus B ) was detected by RT-PCR. All nine patients presented with fever, six had headache, - seven had vomiting. Neck stiffness indicating meningitis was present in six of the patients. Phylogenetic analysis of partial VP1 - VP4-VP2 genes showed these E-9 strains to be most closely related to E-9 strains detected in CSF from Korea - France in 2005 - 2006. The remaining patients were negative for all other viruses tested. E-9 was the most common cause of acute meningoencephalitis in the tested paediatric population from Sri Lanka in 2009, which likely reflects circulation of this E-9 strain between Europe - Asia over several years.
Palavras-Chave: Sri Lanka; Children; Echovirus 9; Enterovirus; Meningoencephalitis
Imprenta: Journal of Medical Virology, v. 87, n. 12, p. 2033-2039, 2015
Identificador do objeto digital: 10.1002/jmv.24267
Descritores: Chikungunya virus - PCR detection ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue
Data de publicação: 2015
