Autor(es): Wintachai Phitchayapak, Kaur Parveen, Lee Regina Ching Hua, Ramphan Suwipa, Kuadkitkan Atichat, Wikan Nitwara, Ubol Sukathida, Roytrakul Sittiruk, Chu Justin Jang Hann, Smith Duncan R

Resumo: Chikungunya virus (CHIKV) is a re-emerging mosquito-borne alphavirus that has recently engendered large epidemics around the world. There is no specific antiviral for treatment of patients infected with CHIKV, - development of compounds with significant anti-CHIKV activity that can be further developed to a practical therapy is urgently required. -rographolide is derived from -rographis paniculata, a herb traditionally used to treat a number of conditions including infections. This study sought to determine the potential of -rographolide as an inhibitor of CHIKV infection. -rographolide showed good inhibition of CHIKV infection - reduced virus production by approximately 3log10 with a 50% effective concentration (EC50) of 77 ?M without cytotoxicity. Time-of-addition - RNA transfection studies showed that -rographolide affected CHIKV replication - the activity of -rographolide was shown to be cell type independent. This study suggests that -rographolide has the potential to be developed further as an anti-CHIKV therapeutic agent.

Palavras-Chave: Alphaviruses; Microbiology techniques

Imprenta: Scientific Reports, v. 5, p. 14179, 2015

Identificador do objeto digital: 10.1038/srep14179

Descritores: Chikungunya virus - Cell ; Chikungunya virus - RNA ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Epidemic

Data de publicação: 2015

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