Active infection of human blood monocytes by Chikungunya virus triggers an innate immune response
Autor(es): Her Zhisheng, Malleret Benoit, Chan Monica, Ong Edward K S, Wong Siew-Cheng, Kwek Dyan J C, Tolou Hugues, Lin Raymond T P, Tambyah Paul Anantharajah, Rénia Laurent, Ng Lisa F P
Resumo: Chikungunya virus (CHIKV) is an alphavirus that causes chronic - incapacitating arthralgia in humans. To date, interactions between the immune system - the different stages of the virus life cycle remain poorly defined. We demonstrated for the first time that CHIKV Ags could be detected in vivo in the monocytes of acutely infected patients. Using in vitro experimental systems, whole blood - purified monocytes, we confirmed that monocytes could be infected - virus growth could be sustained. CHIKV interactions with monocytes, - with other blood leukocytes, induced a robust - rapid innate immune response with the production of specific chemokines - cytokines. In particular, high levels of IFN-alpha were produced rapidly after CHIKV incubation with monocytes. The identification of monocytes during the early phase of CHIKV infection in vivo is significant as infected monocyte/macrophage cells have been detected in the synovial tissues of chronically CHIKV-infected patients, - these cells may behave as the vehicles for virus dissemination. This may explain the persistence of joint symptoms despite the short duration of viremia. Our results provide a better underst-ing on the basic mechanisms of infection - early antiviral immune responses - will help in the development of future effective control strategies.
Imprenta: The Journal of Immunology, v. 184, n. 10, p. 5903-5913, 2010
Identificador do objeto digital: 10.4049/jimmunol.0904181
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Cytokines ; Chikungunya virus - Immune response ; Chikungunya virus - Inflammation ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2010
