A virus-like particle vaccine for epidemic Chikungunya virus protects nonhuman primates against infection
Autor(es): Akahata Wataru, Yang Zhi-Yong, Andersen Hanne, Sun Siyang, Holdaway Heather A, Kong Wing-Pui, Lewis Mark G, Higgs Stephen, Rossmann Michael G, Rao Srinivas, Nabel Gary J
Resumo: Chikungunya virus (CHIKV) has infected millions of people in Africa, Europe - Asia since this alphavirus reemerged from Kenya in 2004. The severity of the disease - the spread of this epidemic virus present a serious public health threat in the absence of vaccines or antiviral therapies. Here, we describe a new vaccine that protects against CHIKV infection of nonhuman primates. We show that selective expression of viral structural proteins gives rise to virus-like particles (VLPs) in vitro that resemble replication-competent alphaviruses. Immunization with these VLPs elicited neutralizing antibodies against envelope proteins from alternative CHIKV strains. Monkeys immunized with VLPs produced high-titer neutralizing antibodies that protected against viremia after high-dose challenge. We transferred these antibodies into immunodeficient mice, where they protected against subsequent lethal CHIKV challenge, indicating a humoral mechanism of protection. Immunization with alphavirus VLP vaccines represents a strategy to contain the spread of CHIKV - related pathogenic viruses in humans.
Palavras-Chave: Immunization; Vaccines; Viral infection
Imprenta: Nature Medicine, v. 16, n. 3, p. 334-338, 2010
Identificador do objeto digital: 10.1038/nm.2105
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Inflammation ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Epidemic ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2010
