A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients.
Autor(es): Goh Lucas Y H, Kam Yiu-Wing, Metz Stefan W, Hobson-Peters Jody, Prow Natalie A, McCarthy Suzi, Smith David W, Pijlman Gorben P, Ng Lisa F P, Hall Roy A
Resumo: Chikungunya fever (CHIKF) has re-emerged as an arboviral disease that mimics clinical symptoms of other diseases such as dengue, malaria, as well as other alphavirus-related illnesses leading to problems with definitive diagnosis of the infection. Herein we describe the development - evaluation of a sensitive epitope-blocking ELISA (EB-ELISA) capable of specifically detecting anti-chikungunya virus (CHIKV) antibodies in clinical samples. The assay uses a monoclonal antibody (mAb) that binds an epitope on the E2 protein of CHIKV - does not exhibit cross-reactivity to other related alphaviruses. We also demonstrated the use of recombinant CHIK virus-like particles (VLPs) as a safe alternative antigen to infectious virions in the assay. Based on testing of 60 serum samples from patients in the acute or convalescent phase of CHIKV infection, the EB-ELISA provided us with 100% sensitivity, - exhibited 98.5% specificity when Ross River virus (RRV)- or Barmah Forest virus (BFV)-immune serum samples were included. This assay meets the public health dem-s of a rapid, robust, sensitive - specific, yet simple assay for specifically diagnosing CHIK-infections in humans.
Palavras-Chave: Chikungunya virus; Clinical diagnostic; Epitope-blocking ELISA; Monoclonal antibodies; Virus-like particles
Imprenta: Journal of Virological Methods, v. 222, p. 55-61, 2015
Identificador do objeto digital: 10.1016/j.jviromet.2015.05.011
Descritores: Chikungunya virus - Cytopathology ; Chikungunya virus - Antibodies ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2015
