A rodent model of Chikungunya virus infection in RAG1 -/- Mice, with features of persistence, for vaccine safety evaluation
Autor(es): Seymour Robert L, Adams A Paige, Leal Grace, Alcorn Maria D H, Weaver Scott C
Resumo: Chikungunya virus (CHIKV) is a positive sense, single str-ed RNA virus in the genus Alphavirus, - the etiologic agent of epidemics of severe arthralgia in Africa, Asia, Europe -, most recently, the Americas. CHIKV causes chikungunya fever (CHIK), a syndrome characterized by rash, fever, - debilitating, often chronic arthritis. In recent outbreaks, CHIKV has been recognized to manifest more neurologic signs of illness in the elderly - those with co-morbidities. The syndrome caused by CHIKV is often self-limited; however, many patients develop persistent arthralgia that can last for months or years. These characteristics make CHIKV not only important from a human health st-point, but also from an economic st-point. Despite its importance as a reemerging disease, there is no licensed vaccine or specific treatment to prevent CHIK. Many studies have begun to elucidate the pathogenesis of CHIKF - the mechanism of persistent arthralgia, including the role of the adaptive immune response, which is still poorly understood. In addition, the lack of an animal model for chronic infection has limited studies of CHIKV pathogenesis as well as the ability to assess the safety of vaccine c-idates currently under development. To address this deficiency, we used recombination activating gene 1 (RAG1-/-) knockout mice, which are deficient in both T - B lymphocytes, to develop a chronic CHIKV infection model. Here, we describe this model as well as its use in evaluating the safety of a live-attenuated vaccine c-idate.
Imprenta: PLoS Neglected Tropical Diseases, v. 9, n. 6, p. e0003800, 2015
Identificador do objeto digital: 10.1371/journal.pntd.0003800
Descritores: Chikungunya virus - Cytopathology ; Chikungunya virus - Immune response ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Immune response ; Chikungunya virus - T lymphocytes ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2015
