A comparison of indirect immunofluorescence and electron microscopy for the diagnosis of some haemorrhagic viruses in cell cultures
Autor(es): El Mekki A A, van der Groen G
Resumo: Yellow fever, dengue (types 1, 2 - 4), Chikungunya, Rift Valley fever, Ebola, Marburg, - Lassa viruses were inoculated into susceptible cell cultures - daily investigated by indirect immunofluorescence (IFA) - electron microscopy (EM) with a view to achieve an early detection-identification of these agents. Compared to the other cell lines tested (Vero, BHK-21 - Aedes albopictus), CV-1 cells were found to be more sensitive. Viral antigens were detected by IFA from a few hours post inoculation (CHIK - RVF) to a maximum of 3 days (YF - EBO). For most of the viruses studied, the cytopathic effect (CPE) commenced 2-3 days after the detection of viral antigens. Virus particles were detected by EM only in the case of EBO, MBG - LAS, before any CPE was observed in cell cultures.
Imprenta: Journal of Virological Methods, v. 3, n. 2, p. 61-69, 1981
Identificador do objeto digital: 10.1016/0166-0934(81)90002-1
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Flaviviridae ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue
Data de publicação: 1981
