2nd International external quality control assessment for the molecular diagnosis of dengue infections
Autor(es): Domingo Cristina, Niedrig Matthias, Teichmann Anette, Kaiser Marco, Rumer Leonid, Jarman Richard G, Donoso-Mantke Oliver
Resumo: Currently dengue viruses (DENV) pose an increasing threat to over 2.5 billion people in over 100 tropical - sub-tropical countries worldwide. International air travel is facilitating rapid global movement of DENV, increasing the risk of severe dengue epidemics by introducing different serotypes. Accurate diagnosis is critical for early initiation of preventive measures. Different reverse transcriptase PCR (RT-PCR) methods are available, which should be evaluated - st-ardized. Epidemiological - laboratory-based surveillance is required to monitor - guide dengue prevention - control programmes, i.e., by mosquito control or possible vaccination (as soon as an effective - safe vaccine becomes available). The purpose of the external quality assurance (EQA) study described is to assess the efficiency - accuracy of dengue molecular diagnosis methods applied by expert laboratories. A panel of 12 human plasma samples was distributed - tested for DENV-specific RNA. The panel comprised 9 samples spiked with different DENV serotypes (DENV-1 to DENV-4), including 10-fold dilution series of DENV-1 - DENV-3. Two specificity controls consisted of a sample with a pool of 4 other flaviviruses - a sample with chikungunya virus. A negative control sample was also included. Thirty-seven laboratories (from Europe, Middle East Asia, Asia, the Americas/Caribbean, - Africa) participated in this EQA study, - reports including 46 sets of results were returned. Performance among laboratories varied according to methodologies used. Only 5 (10.9%) data sets met all criteria with optimal performance, - 4 (8.7%) with acceptable performance, while 37 (80.4%) reported results showed the need for improvement regarding accomplishment of dengue molecular diagnosis. Failures were mainly due to lack of sensitivity - the presence of false positives. The EQA provides information on each laboratory's efficacy of RT-PCR techniques for dengue diagnosis - indicates for most laboratories an urgent need to improve sensitivity - specificity.
Imprenta: PLoS Neglected Tropical Diseases, v. 4, n. 10, 2010
Identificador do objeto digital: 10.1371/journal.pntd.0000833
Descritores: Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - RT-PCRChikungunya Virus - Virus Chikungunya virus - Molecular screening ; Chikungunya virus - Vaccine ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health
Data de publicação: 2010
