Increased levels of the cell cycle inhibitor protein, dacapo, accompany 20-hydroxyecdysone-induced G1 arrest in a mosquito cell line
Autor(es): Gerenday, Anna; Fallon, Ann M.
Resumo: When treated with the steroid hormone 20-hydroxyecdysone (20E), C7-10 cells from the mosquito, Aedes albopictus, arrest in the G1 phase of the cell cycle. To explore whether 20E-mediated cell cycle arrest proceeds through increased levels of cell cycle inhibitor (CKI) proteins, we cloned the Ae. albopictus homolog of dacapo, the single member of the Cip/Kip family of CKI proteins known from Drosophila melanogaster. The Ae. albopictus dacapo cDNA encoded a 261-amino acid homolog of the Aedes aegypti protein XP_001651102.1, which is encoded by an ~23kb gene containing three exons. Like dacapo from D. melanogaster, the ~27kDa protein from Aedes and Culex mosquitoes contained several S/TXXE/D motifs corresponding to potential protein kinase CK2 phosphorylation sites, and a binding site for proliferating cell nuclear antigen (PCNA). When extracts from cells treated with 20E were analyzed by western blotting, using a primary antibody to synthetic peptides from the mosquito dacapo protein, up-regulation of an ~27kDa protein was observed within 24h, and the abundance of the protein further increased by 48h after hormone treatment. This is the first investigation of a cell cycle inhibitory protein in mosquitoes. The results reinforce growing evidence that 20E affects expression of proteins that regulate cell cycle progression. ? 2011 Wiley Periodicals, Inc.
Palavras-Chave: Mosquito; Insect cell line; Antibodies; Antigens; Proteins; Inhibitors; Population dynamics; Steroids; Hormones; Synthetic peptides; Phosphorylation; G1 phase; Cell cycle; Steroid hormones; Dacapo protein
Imprenta: Archives of Insect Biochemistry and Physiology, v. 78, n. 2, p. 61-73, 2011.
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Proteins
Data de publicação: 2011
