Autor(es): Zhang, Dianchang; Ma, Jianjun; Jiang, Jingjing; Qiu, Lihua; Zhu, Caiyan; Su, Tianfeng; Li, Youning; Wu, Kaichang; Jiang, Shigui

Resumo: The lipopolysaccharide and beta-1,3-glucan-binding protein (LGBP) plays an important function in the innate immune response of invertebrates as a pattern recognition receptor (PRR). Herein, we described the isolation and characterization of pearl oyster Pinctada fucata LGBP (designated as poLGBP). The poLGBP cDNA was 2,075 bp long and consisted of a 5'-untranslated region (UTR) of 18 bp, a 3'-UTR of 299 bp with one cytokine RNA instability motifs (ATTTA), and an open reading frame (ORF) of 1,758 bp encoding a polypeptide of 585 amino acids with an estimated molecular mass of 65.1 kDa and a theoretical isoelectric point of 5.80. Homology analysis of the deduced amino acid Sequence of the poLGBP with other known LGBP Sequences by MatGAT software revealed that the poLGBP shared 26.3-56.7% identity and 40.5-70.9% similarity to the other known LGBP Sequences. SMART and alignment analysis revealed that the poLGBP possessed a potential polysaccharide-binding motif, a glucanase motif, a LPS-binding site, a beta-1,3-linkage of polysaccharide, a glycine-rich region, a threonine-rich region and two N-glycosylation sites. In healthy pearl oyster, the poLGBP mRNA was specifically expressed in digestive gland, and not detected in gill, adductor muscle, gonad, intestine, mantle and hemocytes. However, after bacteria stimulation, the expression of the poLGBP mRNA was significantly up-regulated in digestive gland and also weakly detected in haemocytes, gonad and intestine. After LPS stimulation, the poLGBP mRNA expression was significantly up-regulated at 8 and 12 h in digestive gland, and the expression level was 10.7-fold higher than the PBS group at 12 h. After bacteria stimulation, the expression level of the poLGBP mRNA was also significantly up-regulated in digestive gland and was 12.9-fold higher than the PBS group at 8 h. However, during the experiment, the poLGBP mRNA expression was not detected in gill after LPS or bacteria stimulation. The tissue-specific expression and the expression up-regulation after LPS or bacteria stimulation in digestive gland suggested that the poLGBP was an inducible acute-phase protein and might play an important function in digestion as digestive enzyme and pattern recognition receptor.

Palavras-Chave: Pearl oyster; Pinctada fucata; Lipopolysaccharide and beta-1,3-glucan-binding protein; Pattern recognition protein

Imprenta: Molecular Biology Reports, v. 37, n. 7, p. 3335-3343, 2010

Identificador do objeto digital: 10.1007/s11033-009-9920-9

Descritores: Aedes aegypti - Immune response ; Aedes aegypti - Molecular structure ; Aedes aegypti - RNA ; Aedes aegypti - Immunology

Data de publicação: 2010

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