Autor(es): Moshitzky, P; Miloslavski, I; Aizenshtat, Z; Applebaum, SW

Resumo: The corpus allatum (CA) of adult female Ceratitis capitata produces methyl palmitate (NIP) in vitro, in addition to JHB(3) and JH III. Biosynthesized MP migrates on TLC and co-elutes from RP-18 HPLC with synthetic NIP. Its identity is verified herein by GCMS. MP production is up-regulated twofold by mevastatin, an inhibitor of mevalonic acid-dependent isoprene biosynthesis. Fosmidomycin, an inhibitor of mevalonic acid-independent isoprene synthesis in graminaccous plants, up-regulates NIP synthesis by about fourfold. However, it does not depress JHB(3) biosynthesis concurrently. This suggests that the initial enzyme(s) in the conversion of 1-deoxy-xylulose 5-phosphate to isoprene is presumably present in C. capitata, but is inhibited by fosmidomycin, and this inhibition diverts precursors to NIP synthesis. Phytol, an acyclic diterpene, might be suppressing isoprene biosynthesis by CA, thereby resulting in a fourfold increase in the NIP biosynthesis. Linolenic acid is an end-product and its presence in incubation media up-regulates MP biosynthesis by twofold, presumably due to the feedback diversion to biosynthesis of C-16:0 and its methyl ester. Biosynthesis of MP is markedly depressed after mating, while otherwise maintained at significantly higher levels in virgin females. NIP biosynthesis is significantly reduced in virgin females by direct axonal control but is less consistent after mating. (C) 2003 Elsevier Ltd. All rights reserved.

Palavras-Chave: Medfly; Ceratitis Capitata; Corpus Allatum; Methyl Palmitate; Juvenile Hormone; Reproductive Regulation

Imprenta: Insect Biochemistry and Molecular Biology, v. 33, n. 12, p. 1299-1306, 2003

Identificador do objeto digital: 10.1016/j.ibmb.2003.06.008

Descritores: Aedes aegypti - Biosynthesis

Data de publicação: 2003

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