Larvicidal potential of carvacrol and terpinen-4-ol from the essential oil of Origanum vulgare (Lamiaceae) against Anopheles stephensi, Anopheles subpictus, Culex quinquefasciatus and Culex tritaeniorhynchus (Diptera: Culicidae)
Autor(es): Govindarajan, Marimuthu; Rajeswary, Mohan; Hoti, S. L.; Benelli, Giovanni
Resumo: Mosquito-borne diseases represent a deadly threat for millions of people worldwide. However, the use of synthetic insecticides to control Culicidae may lead to resistance, high operational costs and adverse non-target effects. Nowadays, plant-borne mosquitocides may serve as suitable alternative in the fight against mosquito vectors. In this study, the mosquito larvicidal activity of Origanum vulgare (Lamiaceae) leaf essential oil (EO) and its major chemical constituents was evaluated against the malaria vectors Anopheles stephensi and An. subpictus, the filariasis vector Culex quinquefasciatus and the Japanese encephalitis vector Cx. tritaeniorhynchus. The chemical composition of the EO as analyzed by gas chromatography-mass spectroscopy. GC-MS revealed that the essential oil of O. vulgare contained 17 compounds. The major chemical components were carvacrol (38.30%) and terpinen-4-ol (28.70%). EO had a significant toxic effect against early third-stage larvae of An. stephensi, An. subpictus, Cx. quinquefasciatus and Cx. tritaeniorhynchus, with LC50 values of 67.00, 74.14, 8035 and 84.93 mu g/ml. The two major constituents extracted from the O. vulgare EO were tested individually for acute toxicity against larvae of the four mosquito vectors. Carvacrol and terpinen-4-ol appeared to be most effective against An. stephensi (LC50 = 21.15 and 4327 mu g/ml, respectively) followed by An. subpictus (LC50 = 24.06 and 47.73 mu g/ml), Cx. quinquefasciatus (LC50 = 26.08 and 52.19 mu g/ml) and Cx. tritaeniorhynchus (LC50 = 27.95 and 54.87 mu g/ml). Overall, this research adds knowledge to develop newer and safer natural larvicides against malaria, filariasis and Japanese encephalitis mosquito vectors. (C) 2015 Elsevier Ltd. All rights reserved.
Palavras-Chave: Arbovirus; Filariasis; Malaria; Eco-friendly larvicides; Mosquito vectors; Plant-borne mosquitocides
Imprenta: Research in Veterinary Science, v. 104, n. , p. 77-82, 2016
Identificador do objeto digital: 10.1016/j.rvsc.2015.11.011
Descritores: Aedes aegypti - Larvicide ; Aedes aegypti - Repellent
Data de publicação: 2016
