Improvised microinjection technique for mosquito vectors
Autor(es): Kumar, Sampath S.; Puttaraju, H. P.
Resumo: Bio-manipulation technique is of primary importance during the development of transgenic mosquitoes. The study describes the variable factors that influence the viability of medically important mosquito vectors during microinjection.Methods: Three mosquito vectors belonging to the genus Aedes, Anopheles and Culex were microinjected at different developmental stages of their life cycle viz., egg, larvae, pupae and adult.Results: The improvisations revealed an increased survivability of biomanipulated mosquitoes during the embryonic and adult microinjection. The study of injecting larvae and pupae resulted in poor survivability.Interpretation & conclusions: The microinjection protocol was successfully tested on three important mosquito vectors. The critical period after biomanipulation which contributes heavily for the survivability factor was evaluated. The results provide a common protocol for biomanipulation of three mosquito vectors with enhanced survivability.
Palavras-Chave: Bio-Manipulation; Critical Period; Microinjection; Mosquito Vectors; Survivability
Imprenta: Indian Journal of Medical Research v. 136, p. 971-978, 2012
Descritores: Aedes aegypti - Public health
Data de publicação: 2012
