Genetic relationships among Aedes aegypti (Diptera : Culicidae) populations from Argentina using random amplified polymorphic DNA polymerase chain reaction markers
Autor(es): De Sousa, GB; Blanco, A; Gardenal, CN
Resumo: Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) polymorphism was analyzed in five Aedes aegypti (L.) populations from Argentina and one from Puerto Rico to estimate levels of intraspecific polymorphism and genetic relatedness. Allele frequencies were estimated assuming that RAPD products segregate as dominants and that genotype frequencies at those loci are in Hardy-Weinberg equilibrium. Mean expected heterozygosity (H-e) was 0.350: F-ST virtues were significant at all loci except one, supporting the usefulness of the fragments used here to discriminate among populations. Rogers' genetic similarity between samples ranged from 0.806 to 0.621. The population from Puerto Rico was the most different from the Argentina populations. Considering that Atr. aegypti eggs, larvae. and pupae can be transported easily, relationships among the Argentinian populations may reflect the routes and intensity; of commercial transit.
Palavras-Chave: Aedes Aegypti; Random Amplified Polymorphic DNA Polymerase Chain Reaction Markers; Genetic Relatedness; Population Structure
Imprenta: Journal of Medical Entomology, v. 38, n. 3, p. 371-375, 2001
Identificador do objeto digital: 10.1603/0022-2585-38.3.371
Descritores: Aedes aegypti - DNA
Data de publicação: 2001
