Genetic and biological characterization of a densovirus isolate that affects dengue virus infection
Autor(es): Pamplona Mosimann, Ana Luiza; Bordignon, Juliano; Antevere Mazzarotto, Giovanny Camacho; Motta, Maria Cristina M.; Hoffmann, Federico; Duarte dos Santos, Claudia Nunes
Resumo: Brevidensoviruses have an encapsidated, single-stranded DNA genome that predominantly has a negative polarity. In recent years, they have received particular attention due to their potential role in the Biological Control of pathogenic arboviruses and to their unnoticed presence in cell cultures as contaminants. In addition, brevidensoviruses may also be useful as viral vectors. This study describes the first genetic and biological characterization of a mosquito densovirus that was isolated in Brazil; moreover, we examined the phylogenetic relationship between this isolate and the other brevidensoviruses. We further demonstrate that this densovirus has the potential to be used to biologically control dengue virus (DENV) infection with in vitro co-infection experiments. The present study provides evidence that this densovirus isolate is a fast-spreading virus that affects cell growth and DENV infection.
Palavras-Chave: Densovirus; Brevidensovirus; Biological Control; Dengue Virus
Imprenta: Memorias do Instituto Oswaldo Cruz, v. 106, n. 3, p. 285-292, 2011
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Genome
Data de publicação: 2011
