Use of Random Amplified Polymorphic DNA (RAPD) to study genetic diversity within a population of blackfly, Simulium gravelyi from Palni hills, peninsular India
Autor(es): Anbalagan, Sankarappan; Bharathiraja, Chinnapandi; Pandiarajan, Jeyaraj; Dinakaran, Sundaram; Krishnan, Muthukalingan
Resumo: The genetic variation of local subpopulations (Palni hills, India) of the blackfly Simulium gravelyi was surveyed at different altitudinal locations using random amplified polymorphic DNA (RAPD) analysis. By comparing the similarity of the bands produced by RAPDs, it may be concluded that there is variation within this population. These data suggest that the heterogeneity of band pattern of S. gravelyi steadily increased from low elevation to high elevation. Site 5 (1590 m altitude) was more polymorphic and it differed most from site 1 (290 m). This is established by the ecological diversity and statistical analyses (PCA) and predicting two major ecological factors of conductivity and current velocity. The speciation hypothesis is consistent with the molecular evidence, postulates a high elevational site 5 (1590 m) and a subsequent site 4 (1250 m).
Palavras-Chave: Blackfly; Elevation; Diversity; PCA
Imprenta: Biologia, v. 67, n. 6, p. 1195-1203, 2012
Identificador do objeto digital: 10.2478/s11756-012-0104-z
Descritores: Aedes aegypti - DNA
Data de publicação: 2012
