Rapid determination of viral RNA sequences in mosquitoes collected in the field
Autor(es)Kihara, Yuki; Satho, Tomomitsu; Eshita, Yuki; Sakai, Kouji; Kotaki, Akira; Takasaki, Tomohiko; Rongsriyam, Yupha; Komalamisra, Narurnon; Srisawat, Raweewan; Lapcharoen, Parichat; Sumroiphon, Suchada; Iwanaga, Shiroh; Ushijima, Hiroshi; Endoh, Daiji; Miyata, Takeshi; Sakata, Akira; Kashige, Nobuhiro; Miake, Fumio; Fukushi, Shuetsu; Saijo, Masayuki; Kurane, Ichiro; Morikawa, Shigeru; Mizutani, Tetsuya
ResumoA method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses. (c) 2007 Published by Elsevier B.V.
Palavras-ChaveRapid determination; Dengue virus type 4; Cell fusing agent virus; Aedes aegypti
ImprentaJournal of Virological Methods, v. 146, n. 42401, p. 372-374, 2007
Identificador do Objeto Digital10.1016/j.jviromet.2007.07.008
DescritoresAedes aegypti - Cell ; Aedes aegypti - Genome ; Aedes aegypti - RNA ; Aedes aegypti - Infectious diseases ; Aedes aegypti - Virus ; Aedes aegypti - Dengue ; Aedes aegypti - Epidemiology ; Aedes aegypti - Public health
Data de Publicação:2007
