Plasmodium berghei: Effect of protease inhibitors during gametogenesis and early zygote development
Autor(es): Torres, JA; Rodriguez, MH; Rodriguez, MC; Hernandez-Hernandez, FD
Resumo: Plasmodium berghei: The effect of five protease inhibitors, TPCK, TLCK, PMSF, leupeptin, and 1,10-phenanthroline on in vitro gametogenesis and early zygote development of P. berghei was investigated. PMSF and leupeptin showed no effect. Cysteine/serine protease inhibitors TPCK/TLCK at concentrations of 75 and 100 mu M were effective on inhibiting exflagellation center fort-nation, and this effect was reversible with the addition Of L-cysteine. Exflagellation center formation was most effectively blocked by 1,10-phenanthroline (1 mM), and exflagellation center numbers were restored by the addition of Zn2+. A reduction of ookinete production was observed when TPCK/TLCK (100 mu M) was added at 2 h after gametogenesis, but no effect was observed with 1,10-phenanthroline (1 mM). Our results suggest that proteolysis is important in both gametocyte activation and sexual development of P. berghei. (c) 2005 Elsevier Inc. All rights reserved.
Palavras-Chave: Falciparum cysteine protease; Susceptible Aedes aegypti; Malaria parasite; Transmission; Gallinaceum; Identification; Proteinases; Mosquitos; Invasion; Midgut
Imprenta: Experimental Parasitology, v. 111, n. 4, p. 255-259, 2005
Identificador do objeto digital: 10.1016/j.exppara.2005.08.003
Descritores: Aedes aegypti - Sexual
Data de publicação: 2005
