Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti
Autor(es): Sparks, Jackson T.; Dickens, Joseph C.
Resumo: Electrophysiological recording of action potentials from sensory neurons of mosquitoes provides investigators a glimpse into the chemical perception of these disease vectors. We have recently identified a bitter sensing neuron in the labellum of female Aedes aegypti that responds to DEET and other repellents, as well as bitter quinine, through direct electrophysiological investigation. These gustatory receptor neuron responses prompted our sequencing of total mRNA from both male and female labella and tarsi samples to elucidate the putative chemoreception genes expressed in these contact chemoreception tissues. Samples of tarsi were divided into pro-, meso-and metathoracic subtypes for both sexes. We then validated our dataset by conducting qRT-PCR on the same tissue samples and used statistical methods to compare results between the two methods. Studies addressing molecular function may now target specific genes to determine those involved in repellent perception by mosquitoes. These receptor pathways may be used to screen novel repellents towards disruption of host-seeking behavior to curb the spread of harmful viruses.
Palavras-Chave: Molecular Biology; Issue 94; Gustation; Insect; Aedes aegypti; Electrophysiology; Mosquito; RNA-seq; qRT-PCR; Taste; Chemosensory
Imprenta: JOVE-Journal of Visualized Experiments, v. , n. 94, 2014
Identificador do objeto digital: 10.3791/52088
Descritores: Aedes aegypti - Molecular screening ; Aedes aegypti - Repellent
Data de publicação: 2014
