Pharmacological regulation of digestion in the anautogenous flesh fly, Sarcophaga crassipalpis, by simple injection of 6-hydroxydopamine
Autor(es)Bil, Magdalena; Huybrechts, Roger
ResumoFemale anautogenous Sarcophaga flesh flies need a protein meal to start large-scale yolk polypeptides (YPs) production and oocyte maturation. Protein meal rapidly elicits a brain-dependent increase in midgut proteolytic activity. Trypsin and chymotrypsin together represent over 80% of protease activity in liver-fed flies. Abdominal injection of 6-hydroxydopamine (6-OHDA) dose-dependently prohibits this increase in proteolytic activity at translational level in a similar way as post liver feeding decapitation. Delayed injection of 6-OHDA later than 6 h post liver meal has no effect. In flesh flies, chemical decapitation by 6-OHDA, by interrupting the brain-gut dopaminergic signaling, can be used as tool for the controlled inhibition of midgut proteolytic activity and subsequent ovarial development. Inhibition of ovarial development is probably indirect due to a deficit in circulating amino acids needed for YPs synthesis. (C) 2015 Wiley Periodicals, Inc.
Palavras-Chave6-hydroxydopamine; Oxidopamine; Digestion; Vitellogenesis
ImprentaArchives of Insect Biochemistry and Physiology , v. 91, n. 3, p. 137-151, 2016
Identificador do Objeto Digital10.1002/arch.21314
DescritoresAedes aegypti - Protein synthesis
Data de Publicação:2016
