Over-transcription of genes in a parathion-resistant strain of mosquito Culex pipiens quinquefasciatus
Autor(es): Wang, Wei; Liu, Si-Lu; Liu, Yang-Yang; Qiao, Chuan-Ling; Chen, Shao-Liang; Cui, Feng
Resumo: Insecticide resistance is an evolutionary adaptation that develops quite quickly in mosquitoes because of the high selection pressure of chemical insecticides, rapid generation time and large population size. Identification of genes associated with insecticide resistance is fundamental to understand the complex processes responsible for resistance. We compared the gene transcriptional profiles of parathion-resistant and -susceptible Culex pipiens quinquefasciatus using a combination of suppression subtractive hybridization and complementary DNA (cDNA) microarray techniques. A total of 278colonies were selected from the resistant-susceptible mosquito subtractive library, 38 of which showed more than two fold stronger immunoblotting signals in the resistant strain than in the susceptible strain using cDNA microarray selection. The sequencing results showed that the 38colonies can be matched to 12 genes of C. p. quinquefasciatus. Eight genes were confirmed to be overexpressed by more than two fold in the resistant strain. These genes encode chymotrypsin-1, theta glutathione S-transferase, lipase 3, larval serum protein 1 chain, cytochrome b, mitochondrial ribosomal large subunit, 28S rRNA, and a protein with unknown function. This study serves as a preliminary attempt to identify new genes associated with organophosphate resistance in this mosquito species and provides insights into the complicated physiological phenomenon of insecticide resistance.
Palavras-Chave: cDNA microarray; Chymotrypsin; Gene up-regulation; Insecticide resistance; Suppression subtractive hybridization; Theta glutathione S-transferase
Imprenta: Insect Science, v. 22, n. 1, p. 150-156, 2015
Identificador do objeto digital: 10.1111/1744-7917.12106
Descritores: Aedes aegypti - DNA
Data de publicação: 2015
