Comparative analysis of hemocyte phagocytosis between six species of arthropods as measured by flow cytometry
Autor(es): Oliver, Jonathan D.; Loy, J. Dusty; Parikh, Grishma; Bartholomay, Lyric
Resumo: Phagocytosis of pathogens by hemocytes is a rapid-acting immune response and represents a primary means of limiting microbial infection in some species of arthropods. To survey the relative capacity of hemocyte phagocytosis as a function of the arthropod immune response, we examined the extent of phagocytosis among a wide taxonomic range of arthropod species including a decapod crustacean (Litopenaeus vannamei), three ixodid tick species (Amblyomma americanum, Dermacentor variabilis, and lxodes scapularis), a mosquito species (Aedes aegypti), and a larval moth (Manduca sexta). Injected fluorescent beads were used as a model to elicit phagocytosis and were measured by flow cytometry, a technique provided in detail that may be adapted for use with any species of arthropod. The data indicated that smaller arthropods generally had a higher proportion of phagocytic cells than larger arthropods. (C) 2011 Elsevier Inc. All rights reserved.
Palavras-Chave: Flow cytometry; Immune response; Immunity; Hemocyte; Phagocytosis
Imprenta: Journal of Invertebrate Pathology, v. 108, n. 2, p. 126-130, 2011
Identificador do objeto digital: 10.1016/j.jip.2011.07.004
Descritores: Aedes aegypti - Immune response ; Aedes aegypti - Immunology ; Aedes aegypti - Infectious diseases
Data de publicação: 2011
